Simpler & smaller: a new synthetic nanofactory inspired by nature
Bacteria across our planet contain nanometer-sized factories that do many different things. Some make nutrients, others isolate toxic materials that could harm the bacteria. We have barely scratched the surface of their functional diversity.
But all share a common exterior, a shell made of expand iconprotein tiles, that we are learning how to manipulate in the lab. This will allow us to build factories of our own design, using these natural building blocks. Indeed, scientists see these structures as a source of new technologies. They are trying to repurpose them to do things they don’t in nature.
In a new study, the lab of Cheryl Kerfeld reports a new genetically engineered shell, based on natural structures and the principles of protein evolution. The new shell is simpler, made of only a single designed protein. It will be easier to work with and, perhaps, even evolve in the lab. The study is published in ACS Synthetic Biology.
Natural shells are made of up to three types of proteins. The most abundant is called BMC-H. Six BMC-H proteins come together to form a hexagon shape to tile the wall.
At some time in evolutionary history, some pairs of BMC-H proteins became joined together, in tandem. Three of these mergers, called BMC-T, join to also form a hexagon shape.
“The two halves of a BMC-T protein can evolve separately while staying next to each other, because they are fused together. This evolution allows for diversity in the structures and functions of BMC-T shell proteins, something that we want to recreate by design in the lab,” says Bryan Ferlez, a postdoc in the Kerfeld lab.
Taking their cue from this natural evolution of shell proteins, the team created an artificial BMC-T protein, called BMC-H2, by fusing two BMC-H protein sequences together. The new design was successful.
“To our surprise, BMC-H2 proteins form shells on their own. They look like wiffle balls, with gaps in the shell,” says Sean McGuire a former undergraduate research student and technician in the Kerfeld lab. This is because natural shells are icoshedral, meaning that they are made of expand iconhexamer and expand iconpentamers — think of a soccer ball.
Next, the team capped the gaps in the wiffle ball shell with BMC-P, the third type of shell protein that forms pentamers.
“The result is a shell, about 25 nanometers wide, made up of only two protein types: the new BMC-H2 and BMC-P,” Bryan says. “It is around half the size of the structure built with all three protein types.”
The next goal is to fit it with custom expand iconenzymes and fine tune it to enhance the chemical reactions within. The new ‘designer’ shell could have uses in biofuel production, medicine, and industrial applications.
This work was primarily funded by the National Institutes of Health, National Institute of Allergy and Infectious Diseases, and the US Department of Energy, Office of Basic Energy Sciences.
Share this story
The four-year, $898,946 grant from the National Science Foundation will allow Sharkey to continue his research on the evolutionary pattern of the appearance and loss of isoprene emission among various land plants and the impact of these emissions have on the atmosphere.
This long-from article details how our scientists are working to unlock the secrets of photosynthesis, an effort which might spur an agricultural revolution and lead to innovative energy and industrial technologies. The article appears in Futures, a magazine produced twice per year by Michigan State University AgBioResearch.
MSU plant biologist Berkley Walker is part of a team of scientists that is using a 3-year, $1.4 million National Science Foundation Molecular and Cellular Biosciences award to explore the intersection between photorespiration and one-carbon metabolism, two plant biochemical processes that are critical to plant growth and human nutrition.