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Brandon Rohnke (Montgomery lab)

Date & Location: March 17, 2020, at 12p; Room 168 Plant Biology Building

Subject: Connecting the carbon concentrating mechanism to redox regulation in the cyanobacterium Fremyella diplosiphon

Abstract: The carbon concentrating mechanism (CCM) is essential for photoautotrophic growth in cyanobacteria under ambient CO2 levels. Its regulation has been shown to be sensitive to many environmental factors that impact photosynthesis, and some relevant CCM proteins have been shown to be regulated by redox state. One of these, CcmM, is important for Rubisco nucleation and has been shown to have redox-regulated carbonic anhydrase activity in some, but not all, organisms. Fremyella diplosiphon CcmM has the redox-regulated features, and I will discuss hypotheses related to a C194S mutation that could impact redox sensitivity of CcmM. Additionally, the redox state of the plastoquinone pool is an important signal for regulation of photosynthetic proteins.I will discuss progress evaluating the expression level of redox-sensitive reporter genes as well as genes related to non-photochemical quenching under numerous environmental conditions where the carboxysome morphology and CCM gene regulation patterns are known.

Speaker Lab: Dr. Beronda Montgomery