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Dae Kwan Ko (Brandizzi lab)

Date & Location: February 18, 2020, at 12p; Room 168 Plant Biology Building

Subject: A temporal hierarchy underpins the transcription factor-DNA interactome of the maize UPR

Abstract: Adverse environmental conditions reduce crop productivity and often increase the load of unfolded or misfolded proteins in the endoplasmic reticulum (ER). This potentially lethal condition, known as ER stress, is buffered by the unfolded protein response (UPR), a set of signaling pathways designed to either recover ER functionality or ignite programmed cell death. Despite the biological significance of the UPR to the life of the organism, the regulatory transcriptional landscape underpinning ER stress management is largely unmapped, especially in crops.

To fill this significant knowledge gap, we performed a large-scale systems-level analysis of protein-DNA interaction (PDI) network in maize. Using a high-throughput yeast-one hybrid assay with 23 promoter fragments of six UPR marker genes, we identified a highly interconnected network of 262 transcription factors (TFs) associated with significant biological traits and 831 PDIs. We established a temporal hierarchy of TF binding to gene promoters within the same family as well as across different families of TFs. The results in our work are foundational for understanding transcription regulatory mechanisms in the stress responses and crop improvement. 

Speaker Lab: Dr. Federica Brandizzi