Yang-Tsung Ling (Benning Lab)
- Dec 3, 2019
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Date & Location: December 3, 2019, at 12p; Room 168 Plant Biology Building
Subject: Mis-regulation of the transcriptome in the cht7 chlamydomonas mutant during cell division cycle and nitrogen-starvation induced quiescence
Abstract: Control of the cell cycle is directly linked to cell differentiation in multicellular organisms, but in unicellular organisms like microalgae, the progression through the appropriate stages of the cell cycle is critical for survival in a changing environment. Depending on the surrounding environmental factors such as light and nutrients, an algal cell needs to decide whether to undergo cell division or enter a resting (quiescent) state. In the model organism Chlamydomonas, most cell cycle studies have focused on the regulatory mechanism of the cell division cycle and metabolic remodeling during nutrient deprivation-induced quiescence. However, the link between the two states, especially how Chlamydomonas programs the expression of cell cycle genes to transition between cell division and quiescence, is largely unknown.
Recently, a regulator of quiescence transition in Chlamydomonas, Compromised Hydrolysis of TAG 7 (CHT7), was identified in the Benning lab. Subsequent work has shown that CHT7 is required for cells to survive during quiescence. To further investigate the cell cycle control mechanism of CHT7 in Chlamydomonas, we performed an RNA-Sequencing experiment to investigate the transcriptomic dynamics of synchronized cht7 cells at different stages of the cell cycle. The preliminary data indicate that many cell cycle-related genes are derepressed in the cht7 mutant during both the cell division cycle and during quiescence, suggesting CHT7 plays the role of a repressor of these genes. The data also revealed that a group of MAPK genes was highly-induced in the absence of CHT7, implying a possible role of MAPK in cell cycle regulation of Chlamydomonas. A further characterization of these potential CHT7-targeted genes will provide more insight into the regulation of life cycle transitions in microalgae.
Speaker Lab: Dr. Christoph Benning